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Acta cir. bras ; 30(2): 94-99, 02/2015. graf
Article in English | LILACS | ID: lil-741029

ABSTRACT

PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression. .


Subject(s)
Animals , Male , Burns/radiotherapy , Low-Level Light Therapy/methods , Muscle, Skeletal/radiation effects , MyoD Protein/analysis , Burns/metabolism , Burns/pathology , Cell Count , Disease Models, Animal , Immunohistochemistry , Muscle Fibers, Skeletal/radiation effects , Muscle, Skeletal/pathology , MyoD Protein/radiation effects , Rats, Wistar , Reproducibility of Results , Skin/injuries , Skin/radiation effects , Time Factors , Treatment Outcome
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